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rpmi b 27 ins mediumwith small molecule wnt inhibitor iwp2  (Tocris)


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    Tocris rpmi b 27 ins mediumwith small molecule wnt inhibitor iwp2
    Fig. 2 Transcriptome analysis of differentiated hiPSCs (IMR90) toward CMs. The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, <t>IWP2</t> (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. 1A). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.
    Rpmi B 27 Ins Mediumwith Small Molecule Wnt Inhibitor Iwp2, supplied by Tocris, used in various techniques. Bioz Stars score: 97/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi b 27 ins mediumwith small molecule wnt inhibitor iwp2/product/Tocris
    Average 97 stars, based on 512 article reviews
    rpmi b 27 ins mediumwith small molecule wnt inhibitor iwp2 - by Bioz Stars, 2026-03
    97/100 stars

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    1) Product Images from "Transcriptome-based prediction of drugs, inhibiting cardiomyogenesis in human induced pluripotent stem cells."

    Article Title: Transcriptome-based prediction of drugs, inhibiting cardiomyogenesis in human induced pluripotent stem cells.

    Journal: Cell death discovery

    doi: 10.1038/s41420-023-01616-6

    Fig. 2 Transcriptome analysis of differentiated hiPSCs (IMR90) toward CMs. The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, IWP2 (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. 1A). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.
    Figure Legend Snippet: Fig. 2 Transcriptome analysis of differentiated hiPSCs (IMR90) toward CMs. The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, IWP2 (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. 1A). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.

    Techniques Used: Cell Culture, Control



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    rpmi b 27 ins mediumwith small molecule wnt inhibitor iwp2  (Tocris)
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    Tocris rpmi b 27 ins mediumwith small molecule wnt inhibitor iwp2
    Fig. 2 Transcriptome analysis of differentiated hiPSCs (IMR90) toward CMs. The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, <t>IWP2</t> (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. 1A). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.
    Rpmi B 27 Ins Mediumwith Small Molecule Wnt Inhibitor Iwp2, supplied by Tocris, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rpmi b 27 ins mediumwith small molecule wnt inhibitor iwp2/product/Tocris
    Average 97 stars, based on 1 article reviews
    rpmi b 27 ins mediumwith small molecule wnt inhibitor iwp2 - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier
    molecule wnt inhibitor iwp2  (Tocris)
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    Tocris molecule wnt inhibitor iwp2
    The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to <t>Wnt</t> inhibitor, <t>IWP2</t> (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. ). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.
    Molecule Wnt Inhibitor Iwp2, supplied by Tocris, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/molecule wnt inhibitor iwp2/product/Tocris
    Average 97 stars, based on 1 article reviews
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    Fig. 2 Transcriptome analysis of differentiated hiPSCs (IMR90) toward CMs. The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, IWP2 (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. 1A). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.

    Journal: Cell death discovery

    Article Title: Transcriptome-based prediction of drugs, inhibiting cardiomyogenesis in human induced pluripotent stem cells.

    doi: 10.1038/s41420-023-01616-6

    Figure Lengend Snippet: Fig. 2 Transcriptome analysis of differentiated hiPSCs (IMR90) toward CMs. The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, IWP2 (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. 1A). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.

    Article Snippet: The medium was then changed to basal RPMI/B-27-ins medium and cells were kept for further 24 h. At day 2, RPMI/B-27-ins mediumwith small molecule WNT inhibitor IWP2 (Tocris, United Kingdom) 5 μM was added and cells were kept for 48 h (day 2 to day4).

    Techniques: Cell Culture, Control

    The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, IWP2 (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. ). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.

    Journal: Cell Death Discovery

    Article Title: Transcriptome-based prediction of drugs, inhibiting cardiomyogenesis in human induced pluripotent stem cells

    doi: 10.1038/s41420-023-01616-6

    Figure Lengend Snippet: The hiPSCs were cultured as a monolayer on matrigel-coated plates for 2 days under pluripotent conditions and on day 0 exposed to GSK3 inhibitor, CHIR (10 µM) for 24 h. After 48 h exposed to Wnt inhibitor, IWP2 (5 µM). Spontaneously beating cardiac clusters were observed from day 9 onwards. Simultaneously, cells were exposed to test substances for a single exposure of 24 h (day1). The cells were harvested for gene array analysis on day1, day4 and day14 (Fig. ). Medium changes were done as indicated every alternate date. A Representative phase-contrast images of control and ISO treated hiPSC at day1-, 4 -and 14day. Scale bar, 100 µm. B PCA blot of 54,675 probe sets for three timepoints during the differentiation. C PCA blot of the 500 SPS with the highest variance across the mean of the condition‐wise samples. The respective day is indicated by the shape and the respective measured compound is indicated by the color of the dot, as labels are shown next to the plots. The distribution of the data points on the x-axis is given by the PC 1 and on the y-axis by PC2. The percentages in parentheses denote the proportion of explained variance for the respective PC.

    Article Snippet: The medium was then changed to basal RPMI/B-27-ins medium and cells were kept for further 24 h. At day 2, RPMI/B-27-ins medium with small molecule WNT inhibitor IWP2 (Tocris, United Kingdom) 5 μM was added and cells were kept for 48 h (day 2 to day4).

    Techniques: Cell Culture, Control